tm4 mouse sertoli cell line bcrc-60254  (BioResource International Inc)

Journal: Cells

Article Title: Interaction between Chromodomain Y-like Protein and Androgen Receptor Signaling in Sertoli Cells Accounts for Spermatogenesis

doi: 10.3390/cells13100851

Figure Lengend Snippet: AR is colocalized and interacts with CDYL in male testes and Sertoli cells. ( A ) Immunohistochemical staining of AR and CDYL in testes obtained from wild-type and ARKO mice. Bar = 50 um. ( B ) Localization of AR and CDYL (upper) and co-localization of AR, CDYL, and DAPI (bottom) in the testes obtained from wild-type mice by immunofluorescence analysis. Bar = 20 μm. ( C ) Interaction was observed between AR and CDYL in wild-type mouse testes. IgG was used as the control for Western blotting in each group. “Input” means the sample on 10% of volume used for IP. ( D ) Protein expression patterns of AR and CDYL in wild-type mouse testes and ARKO mice by Western blotting. GAPDH was used as the internal control. ( E ) AR and CDYL mRNA expressions were detected in testicular tissues between wild-type and ARKO mice by quantitative RT–PCR assay. ( n ≥ 3) * p ˂ 0.05, by unpaired two-tailed Student t tests was significant compared with the control. Data are expressed as the mean ± standard error of three samples per group. S: Sertoli cell; L: Leydig cell; M: myoid cell; SP: spermatids.

Article Snippet: The TM4 mouse Sertoli cell line (BCRC-60254) was procured from the Bioresource Collection and Research Center (BCRC) in Hsinchu, Taiwan.

Techniques: Immunohistochemical staining, Staining, Immunofluorescence, Control, Western Blot, Expressing, Quantitative RT-PCR, Two Tailed Test

Journal: Cells

Article Title: Interaction between Chromodomain Y-like Protein and Androgen Receptor Signaling in Sertoli Cells Accounts for Spermatogenesis

doi: 10.3390/cells13100851

Figure Lengend Snippet: AR and CDYL regulate downstream AR-targeted genes. ChIP and luciferase assays demonstrate the CDYL ( A ) or TNP1 ( B ) genes in the presence of AR binding sequences. Schematic of a putative binding site for the transcription factor, androgen-responsive elements (ARE) sequences on the CDYL or TNP1 promoter. Promoter occupancy of AR on CDYL or TNP1 promoter by ChIP. CDYL or TNP1 upstream regions starting at position −3000 were introduced into the pGL3-Basic plasmid before the luciferase reporter gene. The siRNA-mediated knockdown ( C ) or overexpression ( D ) of AR or CDYL substantially changed the CDYL or TNP1 promoter activity in TM4 cells through dual-luciferase assays. * p ˂ 0.05 for one-way ANOVA was significant compared with the control. ( n ≥ 3) in each group, and error bars represent ± SD.

Article Snippet: The TM4 mouse Sertoli cell line (BCRC-60254) was procured from the Bioresource Collection and Research Center (BCRC) in Hsinchu, Taiwan.

Techniques: Luciferase, Binding Assay, Plasmid Preparation, Knockdown, Over Expression, Activity Assay, Control

Journal: Cells

Article Title: Interaction between Chromodomain Y-like Protein and Androgen Receptor Signaling in Sertoli Cells Accounts for Spermatogenesis

doi: 10.3390/cells13100851

Figure Lengend Snippet: mRNA expression of AR , CDYL , and TNP1 association by CDYL rescue in Sertoli cells. mRNA expression of AR , CDYL , and TNP1 association among control, AR siRNA-treated, and CDYL siRNA-treated groups in TM4 cells by non-treat and CDYL rescue. mRNA expression of AR ( A , B ), CDYL ( C , D ), or TNP1 ( E , F ) were detected after being transiently transfected with control, AR , or CDYL siRNA-treated ( A , C , E ). Moreover, these three groups were treated with 200 ng CDYL recombinant protein ( B , D , F ) for 24 h in TM4 cells. * p ˂ 0.05 for one-way ANOVA was significant compared with the control. ( n ≥ 3) in each group, and error bars represent ± SD.

Article Snippet: The TM4 mouse Sertoli cell line (BCRC-60254) was procured from the Bioresource Collection and Research Center (BCRC) in Hsinchu, Taiwan.

Techniques: Expressing, Control, Transfection, Recombinant

Journal: Cells

Article Title: Interaction between Chromodomain Y-like Protein and Androgen Receptor Signaling in Sertoli Cells Accounts for Spermatogenesis

doi: 10.3390/cells13100851

Figure Lengend Snippet: Decreased expression of CDYL in the human testes from patients with azoospermia . Immunohistochemistry indicated the expression of AR and CDYL protein in testicular histology from obstructive azoospermia (active spermatogenesis) and non-obstructive azoospermia (defective spermatogenesis), including Sertoli cell-only syndrome (SCOS) and maturation arrest (halted at the primary spermatocyte stage). Bar = 50 μm. The AR and CDYL signals in patients with normal group ( A , D ), Sertoli cell-only syndrome ( B , E ), and maturation arrest ( C , F ) ( n = 1 patient in every group). S: Sertoli cell; L: Leydig cell; M: myoid cell; SP: spermatids.

Article Snippet: The TM4 mouse Sertoli cell line (BCRC-60254) was procured from the Bioresource Collection and Research Center (BCRC) in Hsinchu, Taiwan.

Techniques: Expressing, Immunohistochemistry